Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus
نویسندگان
چکیده
منابع مشابه
Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus.
Four antigenic sites of the E2 glycoprotein of transmissible gastroenteritis virus were defined by competitive radioimmunoassays of monoclonal antibodies (MAbs). Here, we describe the localization of these sites by testing the antigenicity of protein fragments and prokaryotic expression products of E2 gene fragments, and by sequencing of MAb-resistant (mar) mutants. Partial proteolysis of purif...
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Four major antigenic sites have been delineated on the spike protein (S) of the porcine enteric coronavirus transmissible gastroenteritis virus (TGEV) in previous topological studies using monoclonal antibodies (MAbs). Correlation of these sites with the physical structure of the protein was achieved by use of different approaches. Recombinant pEX plasmids directing the synthesis of various fus...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1990
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-71-2-271